We are interested in how free fatty acids (FFAs) regulate diverses cellular fuctions. In particular, our interest is focused on the free fatty acids receptors (FFARs) and, channel and hemichannel formed by connexin (Cx) and pannexin (Panx) proteins.
Our laboratory is part of the Institute of Health Sciences at Universidad de O´Higgins (Rancagua, Chile).
Linolenic acid increase activity of Pannexin 1 through fatty acid receptors in skeletal muscle.
Free fatty acids (FFAs) regulate diverse cellular functions. Also, several free fatty acid receptors (FFARs) have been identified. In particular, FFAR1 (GPR40) and FFAR4 (GPR120) are activated by medium- and long-chain FFAs. Pannexin 1 (Panx1) is a glycoprotein expressed in diverse organs and tissues, such as adult skeletal muscle, and can formed channels permeable to ions and small signaling molecules. We are interested in evaluating the regulation of Panx1 activity induced by fatty acids and FFARs.
Linoleic acid induces a gap closure migration of human oral keratinocytes cells.
The connexin (Cx) proteins form hemichannels (HCs) in the plasma membrane, which are involve in paracrine/autocrine cell signaling. Also, linoleic acid (LA), an essential fatty acid, is known to activate Cx HCs. Moreover, it is reported that Cxs activity is involve in would healing in human oral cell type but possible interlinks between would healing process and Cx HCs activity induced by LA remain unexplored. We evaluated the effect of LA on gap closure migration mediated by Cx HCs in human oral keratinocytes.
Carlos Puebla A., Ph.D
Institute of Health Sciences
Universidad de O´Higgins